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Veal Infusion Broth with Rabbit Serum

[所属分类:培养基配方] [发布时间:2021-10-9] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Veal, infusion from ................................................................... 500.0g

Pancreatic digest of casein............................................................ 5.0g

Peptic digest of animal tissue ....................................................... 5.0g

NaCl.............................................................................................. 5.0g

Rabbit serum, heat inactivated...............................................150.0mL

pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components, except rabbit serum, to

distilled/deionized water and bring volume to 850.0mL. Mix thorough

ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 50°C. Aseptically add 150.0mL of rabbit se

rum. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Boil without agitation prior to use. Use freshly prepared solution or

boil without mixing prior to use.

Use: For the cultivation and maintenance of Proteus mirabilis.