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Veal Infusion Broth with Horse Serum

[所属分类:培养基配方] [发布时间:2021-10-9] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Veal, infusion from ................................................................... 500.0g

Pancreatic digest of casein............................................................ 5.0g

Peptic digest of animal tissue ....................................................... 5.0g

NaCl.............................................................................................. 5.0g

Horse serum, heat inactivated................................................100.0mL

pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components, except horse serum, to

distilled/deionized water and bring volume to 900.0mL. Mix thorough

ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 50°C. Aseptically add 100.0mL of horse se

rum. Mix thoroughly. Aseptically distribute into tubes or flasks. Use

freshly prepared solution or boil without mixing prior to use.

Use: For the cultivation and maintenance of Streptococcus pyogenes.