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Veillonella Agar

[所属分类:培养基配方] [发布时间:2021-10-9] [发布人:网站管理员2] [阅读次数:] [返回]

Veillonella Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Pancreatic digest of casein............................................................ 5.0g

Yeast extract.................................................................................. 3.0g

Sodium thioglycolate .................................................................. 0.75g

Vancomycin ...............................................................................7.5mg

Basic Fuchsin.............................................................................2.0mg

Sodium lactate (60% solution).................................................21.0mL

pH 7.5± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Caution: Basic Fuchsin is a potential carcinogen and care must be

taken to avoid inhalation of the powdered dye and contact with the

skin.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the isolation and cultivation of Veillonella species.