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Vanillate Medium

[所属分类:培养基配方] [发布时间:2021-10-9] [发布人:网站管理员2] [阅读次数:] [返回]

Vanillate Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

(NH4)2SO4..................................................................................... 1.0g

KH2PO4......................................................................................... 0.4g

Yeast extract.................................................................................. 0.1g

MgSO4·7H2O .............................................................................. 0.01g

Trace elements solution ...........................................................10.0mL

Vanillic acid solution ...............................................................10.0mL

Trace Elements Solution:

Composition per liter:

MnSO4·4H2O ................................................................................ 0.4g

H3BO3 ........................................................................................0.5mg

ZnSO4·7H2O ..............................................................................0.4mg

FeCl3 ..........................................................................................0.2mg

(NH4)6Mo7O24·4H2O .................................................................0.2mg

KI ...............................................................................................0.1mg

CuSO4·5H2O ............................................................................0.04mg

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Filter sterilize.

Vanillic Acid Solution:

Composition per 10.0mL:

Vanillic acid, sodium salt .............................................................. 1.5g

Preparation of Vanillic Acid Solution: Add vanillic acid to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components, except vanillic acid so

lution and trace elements solution, to distilled/deionized water and

bring volume to 980.0mL. Mix thoroughly. Gently heat and bring to

boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–

55°C. Warm the vanillic acid solution and the trace elements solution

to 50°–55°C. Aseptically add 10.0mL of sterile vanillic acid solution

and 10.0mL of sterile trace elements solution. Mix thoroughly. Pour

into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Pseudomonas fluorescens.