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Van Niel’s Yeast Medium with Pyruvate, Modified

[所属分类:培养基配方] [发布时间:2021-10-9] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract................................................................................ 10.0g

MgSO4 .......................................................................................... 0.1g

EDTA......................................................................................... 2.0mg

Sodium pyruvate solution......................................................100.0mL

Trace elements solution ...........................................................10.0mL

K2HPO4 solution........................................................................5.0mL

Trace metal A-5 solution ...........................................................1.0mL

pH 7.1± 0.1 at 25°C

Sodium Pyruvate Solution:

Composition per 100.0mL:

Sodium pyruvate........................................................................... 1.1g

Preparation of Sodium Pyruvate Solution: Add sodium pyru

vate to distilled/deionized water and bring volume to 100.0mL. Mix

thoroughly. Filter sterilize.

Trace Elements Solution:

Composition per 100.0mL:

CaCl2·2H2O .................................................................................. 0.3g

Ferric ammonium citrate............................................................... 0.2g

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 100.0mL. Mix thorough

ly. Filter sterilize.

K2HPO4 Solution:

Composition per 100.0mL:

K2HPO4......................................................................................... 4.0g

Preparation of K2HPO4 Solution: Add K2HPO4 to distilled/de

ionized water and bring volume to 100.0mL. Mix thoroughly. Filter

sterilize.

Trace Metal A-5 Solution:

Composition per liter:

H3BO3 ......................................................................................... 2.86g

MnCl2·4H2O................................................................................ 1.81g

Na2MoO4·2H2O .......................................................................... 0.39g

ZnSO4·7H2O ............................................................................. 0.222g

CuSO4·5H2O ............................................................................. 0.079g

Co(NO3)2·6H2O ........................................................................ 0.049g

Preparation of Trace Metal A-5 Solution: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components, except sodium pyru

vate, trace elements, K2HPO4, and trace metal A-5 solutions, to dis

tilled/deionized water and bring volume to 884.0mL. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Asepti

cally add 100.0mL of sterile sodium pyruvate solution, 10.0mL of ster

ile trace elements solution, 5.0mL of sterile K2HPO4 solution, and

1.0mL of sterile trace metal A-5 solution. Mix thoroughly. Adjust pH

to 7.1 ± 0.1. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of photosynthetic bacteria,

such as Heliobacillus mobilis and Rhodopseudomonas palustris.

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Van Niel’s Yeast Medium with Pyruvate, Modified