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VCR Medium

[所属分类:培养基配方] [发布时间:2021-10-9] [发布人:网站管理员2] [阅读次数:] [返回]

VCR Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1001.0mL:

Agar, noble.................................................................................. 15.0g

NaNO3 .......................................................................................... 2.0g

KH2PO4......................................................................................... 1.5g

K2HPO4......................................................................................... 1.2g

NH4Cl ........................................................................................... 0.5g

MgSO4·7H2O................................................................................ 0.2g

FeCl3 ........................................................................................... 0.01g

CaCl2·2H2O ............................................................................. 15.0mg

CuSO4·5H2O.............................................................................. 1.0mg

Vitamin B12 solution ..................................................................1.0mL

pH 7.2 ± 0.2 at 25°C

Vitamin B12 Solution:

Composition per 10.0mL:

Vitamin B12 ...............................................................................10.0μg

Preparation of Vitamin B12 Solution: Add vitamin B12 to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components, except vitamin B12 so

lution, to distilled/deionized water and bring volume to 1.0L. Mix thor

oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 50°–55°C. Aseptically add 1.0mL of sterile

vitamin B12 solution. Mix thoroughly. Pour into sterile Petri dishes or

distribute into sterile tubes.

Use: For the cultivation of Sphaerobacter thermomethanica.