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Ustilago Complete Broth II

[所属分类:培养基配方] [发布时间:2021-10-8] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose ....................................................................................... 10.0g

Hydrolyzed casein......................................................................... 2.5g

NH4NO3 ........................................................................................ 1.5g

Yeast extract.................................................................................. 1.0g

Salt solution .............................................................................62.5mL

Vitamin solution.......................................................................10.0mL

Hydrolyzed nucleic acids solution.............................................5.0mL

pH 7.0 ± 0.2 at 25°C

Salt Solution:

Composition per liter:

KH2PO4....................................................................................... 16.0g

KCl................................................................................................ 8.0g

Na2SO4.......................................................................................... 4.0g

MgSO4 .......................................................................................... 2.0g

CaCl2............................................................................................. 1.0g

Trace elements solution .............................................................8.0mL

Trace Elements Solution:

Composition per liter:

CuSO4·5H2O ................................................................................. 0.4g

ZnCl2............................................................................................. 0.4g

MnCl2·4H2O................................................................................ 0.14g

FeCl3·6H2O............................................................................ 100.0mg

H3BO3 ...................................................................................... 60.0mg

Na2MoO4·2H2O ....................................................................... 40.0mg

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Salt Solution: Add components to distilled/deion

ized water and bring volume to 1.0L. Mix thoroughly.

Vitamin Solution:

Composition per liter:

Inositol .......................................................................................... 1.0g

Calcium pantothenate ................................................................... 0.2g

Choline chloride............................................................................ 0.2g

Nicotinic acid................................................................................ 0.2g

Thiamine................................................................................ 100.0mg

p-Aminobenzoic acid............................................................... 50.0mg

Pyridoxine................................................................................ 50.0mg

Riboflavin ................................................................................ 50.0mg

Preparation of Vitamin Solution: Add components to distilled/

deionized water and bring volume to 1.0L. Mix thoroughly.

Hydrolyzed Nucleic Acids Solution:

Composition per 80.0mL:

DNA, calf thymus......................................................................... 2.0g

RNA.............................................................................................. 2.0g

HCl (1M solution)....................................................................30.0mL

NaOH (1M solution)................................................................30.0mL

Preparation of Hydrolyzed Nucleic Acids Solution: Add DNA

to 30.0mL of 1M NaOH solution. Add RNA to 30.0mL of 1M HCl so

lution. Autoclave the two solutions separately for 10 min at 15 psi pres

sure–121°C. Mix the two solutions. Adjust the pH to 6.0. Centrifuge at

5000 × g for 10 min. Decant supernatant solution and filter. Bring vol

ume to 80.0mL with distilled/deionized water. Store at −20°C.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0.

Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–

121°C.

Use: For the cultivation of Ustilago species.

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Ustilago Complete Broth II