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Ustilago Medium

[所属分类:培养基配方] [发布时间:2021-10-8] [发布人:网站管理员2] [阅读次数:] [返回]

Ustilago Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract................................................................................ 11.0g

Glucose ....................................................................................... 10.0g

NH4NO3 ........................................................................................ 1.5g

Salt solution .............................................................................62.5mL

Vitamin solution.......................................................................10.0mL

Salt Solution:

Composition per liter:

KH2PO4....................................................................................... 16.0g

KCl................................................................................................ 8.0g

Na2SO4.......................................................................................... 4.0g

MgSO4·7H2O................................................................................ 2.0g

CaCl2............................................................................................. 1.0g

Trace elements solution .............................................................8.0mL

Preparation of Salt Solution: Add components to distilled/deion

ized water and bring volume to 1.0L. Mix thoroughly.

Trace Elements Solution:

Composition per 500.0mL:

CuSO4·5H2O................................................................................. 0.2g

ZnCl2............................................................................................. 0.2g

MnCl2·4H2O................................................................................ 0.07g

FeCl3·6H2O ................................................................................. 0.05g

H3BO3 ......................................................................................... 0.03g

Na2MoO4·2H2O .......................................................................... 0.02g

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 500.0mL. Mix thorough

ly.

Vitamin Solution:

Composition per liter:

Inositol .......................................................................................... 0.4g

Calcium pantothenate ................................................................... 0.2g

Choline chloride............................................................................ 0.2g

Nicotinic acid................................................................................ 0.2g

Thiamine ....................................................................................... 0.1g

Pyridoxine................................................................................... 0.05g

Riboflavin ................................................................................... 0.05g

Preparation of Vitamin Solution: Add components to distilled/

deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster

ilize.

Preparation of Medium: Add components, except vitamin solution,

to distilled/deionized water and bring volume to 990.0mL. Mix thor

oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of sterile

vitamin solution. Mix thoroughly. Aseptically distribute into sterile tubes

or flasks.

Use: For the cultivation of Ustilago species.

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Ustilago Medium