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Ustilago Complete Agar II

[所属分类:培养基配方] [发布时间:2021-10-8] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

Glucose ....................................................................................... 10.0g

Hydrolyzed casein ........................................................................ 2.5g

NH4NO3 ........................................................................................ 1.5g

Yeast extract.................................................................................. 1.0g

Salt solution .............................................................................62.5mL

Vitamin solution.......................................................................10.0mL

Hydrolyzed nucleic acids solution.............................................5.0mL

pH 7.0 ± 0.2 at 25°C

Salt Solution:

Composition per liter:

KH2PO4....................................................................................... 16.0g

KCl................................................................................................ 8.0g

Na2SO4.......................................................................................... 4.0g

MgSO4 .......................................................................................... 2.0g

CaCl2............................................................................................. 1.0g

Trace elements solution .............................................................8.0mL

Trace Elements Solution:

Composition per liter:

CuSO4·5H2O................................................................................. 0.4g

ZnCl2............................................................................................. 0.4g

MnCl2·4H2O ............................................................................... 0.14g

FeCl3·6H2O............................................................................ 100.0mg

H3BO3 ...................................................................................... 60.0mg

Na2MoO4·2H2O ....................................................................... 40.0mg

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Salt Solution: Add components to distilled/deion

ized water and bring volume to 1.0L. Mix thoroughly.

Vitamin Solution:

Composition per liter:

Inositol .......................................................................................... 1.0g

Calcium pantothenate ................................................................... 0.2g

Choline chloride............................................................................ 0.2g

Nicotinic acid................................................................................ 0.2g

Thiamine ................................................................................100.0mg

p-Aminobenzoic acid...............................................................50.0mg

Pyridoxine................................................................................50.0mg

Riboflavin ................................................................................50.0mg

Preparation of Vitamin Solution: Add components to distilled/

deionized water and bring volume to 1.0L. Mix thoroughly.

Hydrolyzed Nucleic Acids Solution:

Composition per 80.0mL:

DNA, calf thymus ......................................................................... 2.0g

RNA .............................................................................................. 2.0g

HCl (1M solution)....................................................................30.0mL

NaOH (1M solution) ................................................................30.0mL

Preparation of Hydrolyzed Nucleic Acids Solution: Add DNA

to 30.0mL of 1M NaOH solution. Add RNA to 30.0mL of 1M HCl so

lution. Autoclave the two solutions separately for 10 min at 15 psi pres

sure–121°C. Mix the two solutions. Adjust the pH to 6.0. Centrifuge at

5000 × g for 10 min. Decant supernatant solution and filter. Bring vol

ume to 80.0mL with distilled/deionized water. Store at −20°C.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Adjust pH to 7.0. Distribute into tubes or flasks. Autoclave

for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or

leave in tubes.

Use: For the cultivation and maintenance of Ustilago species.

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Ustilago Complete Agar II