Urogenital Mycoplasma Broth Base

Urogenital Mycoplasma Broth Base

Heart infusion powder .................................................................. 8.0g

Casein enzymatic hydrolysate ...................................................... 8.0g

Yeast extract.................................................................................. 4.0g

NaCl .............................................................................................. 3.5g

Arginine hydrochloride................................................................. 5.0g

Cysteine hydrochloride ................................................................. 0.1g

Phenol Red.................................................................................. 0.05g

Horse serum ..............................................................................50.0ml

Urea solution............................................................................10.0mL

Vitamin solution.......................................................................10.0mL

Selective supplement solution .................................................10.0mL

pH 6.3 ± 0.2 at 25°C

Source: This medium is available from HiMedia.

Selective Supplement Solution:

Composition per 10.0mL:

Penicillin ....................................................................................5.0mg

Amphotericin B..........................................................................1.0mg

Penicillin .............................................................................. 100,000U

Preparation of Selective Supplement Solution: Add compo

nents to distilled/deionized water and bring volume to 10.0mL. Mix

thoroughly. Filter sterilize.

Urea Solution:

Composition per 10.0mL:

Urea............................................................................................... 0.5g

Preparation of Urea Solution: Add urea to distilled/deionized wa

ter and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Vitamain Solution:

Composition per 10.0mL:

Glucose ......................................................................................... 2.0g

L-Cysteine·HCl ......................................................................... 0.518g

L-Glutamine .................................................................................. 0.2g

L-Cystine................................................................................... 0.022g

Adenine sulfate ........................................................................... 0.02g

Nicotinamide adenine dinucleotide ........................................... 5.0mg

Cocarboxylase............................................................................ 2.0mg

Guanine·HCl .............................................................................. 0.6mg

Fe(NO3)3·6H2O.......................................................................... 0.4mg

p-Aminobenzoic acid............................................................... 0.26mg

Vitamin B12 ................................................................................ 0.2mg

Thiamine·HCl .......................................................................... 0.06mg

Preparation of Vitamin solution: Add components to distilled/

deionized water and bring volume to 10.0mL. Mix thoroughly. Filter

sterilize.

Preparation of Medium: Add components, except vitamin solu

tion, urea solution, horse serum, and selective supplement solution, to

distilled/deionized water and bring volume to 920.0mL. Mix thorough

ly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Asep

tically add vitamin solution, urea solution, horse serum, and selective

supplement solution. Mix thoroughly. Aseptically distribute into sterile

tubes.

Use: For the selective culture of Mycoplasma hominis and Ure

aplasma urealyticum.