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Treponema macrodentium Medium

[所属分类:培养基配方] [发布时间:2021-9-18] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose ......................................................................................... 1.0g

Nicotinamide................................................................................. 0.4g

Spermine·4HCl ........................................................................... 0.15g

Sodium isobutyrate ..................................................................... 0.02g

Carboxylase ...............................................................................5.0mg

PPLO agar..............................................................................900.0mL

Bovine serum .........................................................................100.0mL

pH 7.0 ± 0.2 at 25°C

PPLO Agar:

Composition per 900.0mL:

Beef heart, infusion from ............................................................ 50.0g

Agar ............................................................................................ 14.0g

Peptone........................................................................................ 10.0g

NaCl .............................................................................................. 5.0g

pH 7.8 ± 0.2 at 25°C

Preparation of PPLO Agar: Add components to distilled/deion

ized water and bring volume to 900.0mL. Mix thoroughly.

Preparation of Medium: Combine components, except bovine se

rum. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Cool to 45°–50°C. Aseptically add sterile bovine serum. Mix thor

oughly. Aseptically distribute into sterile tubes or flasks.

Use: For the isolation and cultivation of Treponema macrodentium.