培养基配方

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Treponema Isolation Medium

[所属分类:培养基配方] [发布时间:2021-9-18] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Beef heart, solids from infusion.................................................. 20.0g

Ionagar No. 2 ................................................................................ 7.2g

K2HPO4......................................................................................... 2.0g

Arabinose...................................................................................... 0.8g

Glucose ......................................................................................... 0.8g

Maltose ......................................................................................... 0.8g

Polypeptone™ .............................................................................. 0.8g

Pyruvate ........................................................................................ 0.8g

Starch, soluble............................................................................... 0.8g

Sucrose.......................................................................................... 0.8g

Cysteine·HCl............................................................................... 0.68g

(NH4)2SO4 .................................................................................... 0.6g

Serine ............................................................................................ 0.4g

Tryptose ........................................................................................ 0.4g

Yeast extract.................................................................................. 0.4g

NaCl.............................................................................................. 0.2g

Rumen fluid ...........................................................................500.0mL

Rabbit serum-cocarboxylase solution....................................100.0mL

pH 7.2 ± 0.2 at 25°C

Rabbit Serum-Cocarboxylase Solution:

Composition per liter:

Rabbit serum, heat inactivated...............................................100.0mL

Cocarboxylase solution..............................................................1.0mL

Preparation of Rabbit Serum-Cocarboxylase Solution: Heat

rabbit serum at 56°C for 1 hr. Add 1.0mL of cocarboxylase solution.

Mix thoroughly.

Cocarboxylase Solution:

Composition per 1.0mL:

Cocarboxylase............................................................................... 0.5g

Preparation of Cocarboxylase Solution: Add cocarboxylase to

1.0mL of distilled/deionized water. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except rumen fluid and

rabbit serum-cocarboxylase solution, to distilled/deionized water and

bring volume to 400.0mL. Mix thoroughly. Gently heat and bring to boil

ing. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add 500.0mL of sterile rumen fluid and 100.0mL of sterile

rabbit serum-cocarboxylase solution. Mix thoroughly. Pour into sterile

Petri dishes or distribute into sterile tubes.

Use: For the isolation of oral treponemes.