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Treponema Medium

[所属分类:培养基配方] [发布时间:2021-9-18] [发布人:网站管理员2] [阅读次数:] [返回]

Treponema Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 30.0g

Ionagar No. 2 ................................................................................ 8.0g

Glucose ......................................................................................... 5.0g

Yeast extract.................................................................................. 5.0g

NaCl .............................................................................................. 2.5g

Cysteine·HCl............................................................................... 0.75g

Horse serum, inactivated........................................................100.0mL

pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components, except horse serum, to

distilled/deionized water and bring volume to 900.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Aseptically add 100.0mL of sterile horse se

rum. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile

tubes.

Use: For the isolation and cultivation of oral treponemes.