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Treponema Isolation Medium

[所属分类:培养基配方] [发布时间:2021-9-18] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution A ..............................................................................450.0mL

Spirolate broth........................................................................450.0mL

Rabbit serum,

inactivated at 56°C for 30 min ........................................100.0mL

pH 7.4 ± 0.2 at 25°C

Solution A:

Composition per 450.0mL:

Agar .............................................................................................. 8.0g

Asparagine .................................................................................. 0.25g

Sodium thioglycolate .................................................................. 0.25g

Pancreatic digest of casein.......................................................... 0.25g

Brain heart infusion broth ......................................................450.0mL

Preparation of Solution A: Combine components. Mix thorough

ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C.

Brain Heart Infusion Broth:

Composition per liter:

Pancreatic digest of gelatin......................................................... 14.5g

Brain heart, solids from infusion .................................................. 6.0g

Peptic digest of animal tissue ....................................................... 6.0g

NaCl.............................................................................................. 5.0g

Casein ........................................................................................... 5.0g

Glucose ......................................................................................... 3.0g

Na2HPO4....................................................................................... 2.5g

Preparation of Brain Heart Infusion Broth: Add components

to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Spirolate Broth:

Composition per liter:

Pancreatic digest of casein.......................................................... 15.0g

Glucose ......................................................................................... 5.0g

Yeast extract.................................................................................. 5.0g

NaCl.............................................................................................. 2.5g

L-Cysteine·HCl·H2O ..................................................................... 1.0g

Sodium thioglycolate.................................................................... 0.5g

Palmitic acid ............................................................................... 0.05g

Stearic acid.................................................................................. 0.05g

Oleic acid.................................................................................... 0.05g

Linoleic acid ............................................................................... 0.05g

Preparation of Spirolate Broth: Add components to distilled/de

ionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for

15 min at 15 psi pressure–121°C. Cool to 25°C.

Preparation of Medium: Combine 450.0mL of sterile solution A,

450.0mL of sterile spirolate broth, and 100.0mL of rabbit serum. Mix

thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the isolation and cultivation of oral, genital, and fecal

treponemes.

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Treponema Isolation Medium