Peptococcus glycinophilus Medium

[所属分类:培养基配方] [发布时间:2021-7-1] [发布人:网站管理员2] [阅读次数:] [返回]

Peptococcus glycinophilus Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein............................................................5.0g
Yeast extract..................................................................................5.0g
Glycine..........................................................................................3.0g
Agar ..............................................................................................2.0g
Reducing agent ........................................................................20.0mL
Potassium phosphate buffer (1M, pH 7.1).................................5.0mL
Salts B........................................................................................1.0mL
Reducing Agent:
Composition per 100.0mL:
NaHCO 3 ........................................................................................5.0g
Na 2 S 2 O 4 ........................................................................................1.0g
Preparation of Reducing Agent: Add components to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly.
Salts B:
Composition per 100.0mL:
MgSO 4 ·7H 2 O..............................................................................20.0g
FeSO 4 ·7H 2 O..................................................................................1.0g
MnSO 4 ·H 2 O..................................................................................0.5g
Preparation of Salts B: Add components to distilled/deionized wa-
ter and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation and maintenance of Peptostreptococcus
micros (Peptococcus glycinophilus).