Peptococcus glycinophilus Medium (DSMZ Medium 228)

[所属分类:培养基配方] [发布时间:2021-7-1] [发布人:网站管理员2] [阅读次数:] [返回]

Peptococcus glycinophilus Medium
(DSMZ Medium 228)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract................................................................................10.0g
NaCl..............................................................................................8.0g
Trypticase™ peptone....................................................................5.0g
Peptone .........................................................................................5.0g
Beef extract...................................................................................5.0g
Glycine..........................................................................................3.0g
K 2 HPO 4 .........................................................................................2.0g
L -Cysteine·HCl .............................................................................0.5g
Resazurin ...................................................................................1.0mg
Salt solution.............................................................................40.0mL
Hemin solution.........................................................................10.0mL
Tween™ 80................................................................................1.0mL
Vitamin K 1 solution...................................................................0.2mL
pH 7.2± 0.2 at 25°C
Salt Solution:
Composition per liter:
NaHCO 3 ......................................................................................10.0g
NaCl..............................................................................................2.0g
K 2 HPO 4 .........................................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.5g
CaCl 2 ·2H 2 O ................................................................................0.25g
Preparation of Salt Solution:  Add components to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly.
Vitamin K 1 Solution:
Composition per 20.0mL:
Vitamin K 1 ....................................................................................0.1g
Preparation of Vitamin K 1 Solution:  Add vitamin K 1 to 20.0mL of
95% ethanol. Mix thoroughly. Store refrigerated in a brown bottle.
Hemin Solution:
Composition per 20.0mL:
Hemin ......................................................................................50.0mg
NaOH (1N solution).................................................................20.0mL
Preparation of Hemin Solution:  Add hemin to 1.0mL of 1N
NaOH solution. Mix thoroughly. Bring volume to 20.0mL with dis-
tilled/deionized water. Store refrigerated.
Preparation of Medium: Add components, except  L -cysteine·HCl,
vitamin K 1  solution, and hemin solution, to distilled/deionized water
and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring
to boiling. Sparge with CO 2 . Add  L -cysteine·HCl. Cool to 25°C. Asep-
tically add 10.0mL hemin solution and 0.2mL vitamin K 1 solution.
Mix thoroughly. Adjust pH to 7.2 using 8N NaOH. Distribute into
tubes or flasks under N 2 . Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the cultivation of Micromonas micros=Peptostreptococcus
micros.