Peptococcus Medium

[所属分类:培养基配方] [发布时间:2021-7-1] [发布人:网站管理员2] [阅读次数:] [返回]

Peptococcus Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Casein peptone............................................................................10.0g
Beef extract...................................................................................3.0g
Yeast extract..................................................................................3.0g
Glucose .........................................................................................2.0g
L -Cysteine·HCl..............................................................................0.5g
Salt solution .............................................................................40.0mL
Tween™ 80................................................................................1.0mL
pH 7.2 ± 0.2 at 25°C
Salt Solution:
Composition per liter:
NaHCO 3 ......................................................................................10.0g
NaCl..............................................................................................2.0g
K 2 HPO 4 ........................................................................................1.0g
KH 2 PO 4 ........................................................................................1.0g
CaCl 2 .............................................................................................0.2g
MgSO 4 ·7H 2 O................................................................................0.2g
Preparation of Salt Solution:  Add components to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized wa-
ter and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Distribute
into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation of Gemella morbillorum, Megasphaera elsdenii,
and Streptococcus pleomorphus.