Pept Carb Soluble Starch Agar (Peptone Carbonate Starch Agar)

[所属分类:培养基配方] [发布时间:2021-7-1] [发布人:网站管理员2] [阅读次数:] [返回]

Pept Carb Soluble Starch Agar
(Peptone Carbonate Starch Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Solution A..............................................................................900.0mL
Solution B..............................................................................100.0mL
Solution A:
Composition per 900.0mL:
Soluble starch..............................................................................20.0g
Agar ............................................................................................15.0g
Peptone..........................................................................................5.0g
Yeast extract..................................................................................5.0g
K 2 HPO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.2g
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 900.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 10 psi pressure–115°C. Cool
to 45°–50°C.
Solution B:
Composition per 100.0mL:
Na 2 CO 3  .......................................................................................10.0g
Preparation of Solution B: Add Na 2 CO 3  to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15
min at 10 psi pressure–115°C. Cool to 45°–50°C.
Preparation of Medium: Aseptically combine cooled sterile solu-
tion A with cooled sterile solution B. Mix thoroughly. Aseptically dis-
tribute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Bacillus species.