Pedomicrobium PYVM Medium

[所属分类:培养基配方] [发布时间:2021-7-1] [发布人:网站管理员2] [阅读次数:] [返回]

Pedomicrobium PYVM Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Peptone........................................................................................0.25g
Yeast extract................................................................................0.25g
Modified Hutner’s basal salts ..................................................20.0mL
DL -Malate solution...................................................................10.0mL
Vitamin solution.......................................................................10.0mL
pH 7.5 ± 0.2 at 25°C
Modified Hutner’s Basal Salts:
Composition per liter:
MgSO 4 ·7H 2 O..............................................................................29.7g
Nitrilotriacetic acid.....................................................................10.0g
CaCl 2 ·2H 2 O.................................................................................3.34g
FeSO 4 ·7H 2 O..................................................................................0.1g
(NH 4 ) 2 MoO 4 ............................................................................9.25mg
Metals “44”..............................................................................50.0mL
Preparation of Modified Hutner’s Basal Salts: Add nitrilotria-
cetic acid to 500.0mL of distilled/deionized water. Dissolve by adjust-
ing pH to 6.5 with KOH. Add remaining components. Readjust pH to
7.2 with H 2 SO 4 or KOH. Add distilled/deionized water to 1.0L. Store
at 5°C.
Metals “44”:
Composition per 100.0 mL:
ZnSO 4 ·7H 2 O.................................................................................1.1g
FeSO 4 ·7H 2 O..................................................................................0.5g
EDTA..........................................................................................0.25g
MnSO 4 ·7H 2 O............................................................................0.154g
CuSO 4 ·5H 2 O...............................................................................0.04g
Co(NO 3 ) 2 ·6H 2 O ........................................................................0.025g
Na 2 B 4 O 7 ·10H 2 O........................................................................0.018g
Preparation of Metals “44”: Add a few drops of H 2 SO 4 to dis-
tilled/deionized water to inhibit precipitate formation. Add compo-
nents to acidified distilled/deionized water and bring volume to
100.0mL. Mix thoroughly.
DL -Malate Solution:
Composition per 100.0mL:
DL -Malic acid..............................................................................20.0g
Preparation of  DL -Malate Solution:  Add malic acid to distilled/
deionized water and bring volume to 80.0mL. Mix thoroughly. Adjust
pH to 7.5 with NaOH. Bring volume to 100.0mL with distilled/deion-
ized water. Filter sterilize.
Vitamin Solution:
Composition per liter:
Pyridoxine·HCl........................................................................10.0mg
Riboflavin..................................................................................5.0mg
Nicotinamide..............................................................................5.0mg
p-Aminobenzoic acid.................................................................5.0mg
Thiamine·HCl............................................................................5.0mg
Calcium  D -(+)-pantothenate ......................................................5.0mg
D -(+)-Biotin ...............................................................................2.0mg
Folic acid ...................................................................................2.0mg
Cyanocobalamine ......................................................................0.1mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except  DL -malate solu-
tion and vitamin solution, to distilled/deionized water and bring vol-
ume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add
10.0mL of sterile  DL -malate solution and 10.0mL of sterile vitamin so-
lution. Mix thoroughly. Distribute into sterile tubes or flasks.
Use: For the cultivation of Pedomicrobium americanum.