Pedomicrobium PYVM Medium

[所属分类:培养基配方] [发布时间:2021-7-1] [发布人:网站管理员2] [阅读次数:] [返回]

Pedomicrobium PYVM Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Peptone .......................................................................................0.25g
Yeast extract................................................................................0.25g
Trace elements solution...........................................................20.0mL
Malate solution ........................................................................10.0mL
Vitamin solution.......................................................................10.0mL
pH 7.1 ± 0.2 at 25°C
Malate Solution:
Composition per 100.0mL:
Malic acid...................................................................................1.36g
Preparation of Malate Solution: Add malic acid to distilled/de-
ionized water and bring volume to 100.0mL. Adjust pH to 7.0 with
concentrated NaOH. Mix thoroughly. Filter sterilize.
Vitamin Solution:
Composition per liter:
Pyridoxine·HCl........................................................................10.0mg
Thiamine·HCl ............................................................................5.0mg
Calcium pantothenate ................................................................5.0mg
Riboflavin..................................................................................5.0mg
Nicotinic acid.............................................................................5.0mg
p-Aminobenzoic acid.................................................................5.0mg
D -Biotin......................................................................................2.0mg
Folic acid ...................................................................................2.0mg
Cyanocobalamin........................................................................0.1mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Trace Elements Solution:
Composition per 50.0mL:
ZnSO 4 ·7H 2 O...............................................................................0.55g
FeSO 4 ·7H 2 O................................................................................0.25g
EDTA........................................................................................0.125g
MnSO 4 ·H 2 O..............................................................................0.077g
CuSO 4 ·5H 2 O...............................................................................0.02g
Co(NO 3 ) 2 ·6H 2 O ........................................................................0.012g
Na 2 B 4 O 7 ·10H 2 O.......................................................................8.85mg
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 50.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except malate solution
and vitamin solution, to distilled/deionized water and bring volume to
980.0mL. Mix thoroughly. Adjust pH to 7.1 with KOH. Autoclave for
15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add
10.0mL of sterile malate solution and 10.0mL of sterile vitamin solu-
tion. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation of Pedomicrobium species.