Pectin Agar

[所属分类:培养基配方] [发布时间:2021-6-30] [发布人:网站管理员2] [阅读次数:] [返回]

Pectin Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per plate:

Base agar..................................................................................15.0mL
Pectin gel....................................................................................7.0mL
Base Agar:
Composition per liter:
Agar ............................................................................................15.0g
K 2 HPO 4 .........................................................................................5.0g
CaCl 2 ·2H 2 O...................................................................................3.0g
NH 4 Cl ...........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.2g
Tris(hydroxymethyl)amino–
methane buffer (1M, pH 8.0)...........................................100.0mL
Trace elements solution .............................................................1.0mL
Preparation of Base Agar: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C.
Trace Elements Solution:
Composition per liter:
Disodium EDTA ...........................................................................8.0g
MnCl 2 ·4H 2 O..................................................................................0.1g
CoCl 2 ·6H 2 O ................................................................................0.02g
ZnCl 2 ...........................................................................................0.02g
KBr..............................................................................................0.02g
KI................................................................................................0.02g
CuSO 4 .........................................................................................0.01g
Na 2 MoO 4 ·2H 2 O..........................................................................0.01g
H 3 BO 3 .........................................................................................0.01g
LiCl............................................................................................5.0mg
SnCl 2 ·2H 2 O................................................................................5.0mg
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Pectin Gel:
Composition per liter:
Pectin, low esterified ..................................................................20.0g
Preparation of Pectin Gel: Add pectin to 70°C distilled/deionized
water and bring volume to 1.0L. Autoclave for 10 min at 7 psi pres-
sure–110°C. Cool to 45°–50°C.
Preparation of Medium:  Pour cooled, sterile base agar into sterile
Petri dishes in 15.0mL volumes. Allow agar to solidify. Overlay each
plate with 7.0mL of cooled, sterile pectin gel. The pectin may take 5 hr
to form a gel.
Use: For the isolation and cultivation of Cytophaga species, Herpeto-
siphon species, Saprospira species, and Flexithrix species.