Pectin Medium

[所属分类:培养基配方] [发布时间:2021-6-30] [发布人:网站管理员2] [阅读次数:] [返回]

Pectin Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pectin..........................................................................................30.0g
Yeast extract..................................................................................5.0g
Bromthymol Blue (0.1% solution) ............................................1.0mL
CaCl 2 ·2H 2 O (10.0% solution)....................................................0.6mL
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add 100.0mL of distilled/deionized wa-
ter to a 2.0L flask and place on a magnetic stirrer with no heat. While
stirring, slowly add CaCl 2 ·2H 2 O, Bromthymol Blue solution, yeast ex-
tract, and pectin. Add slowly to ensure each particle is wetted. Gently
heat and bring to almost boiling. Adjust pH to 7.3 with 1N NaOH. Do
not overshoot pH 7.3.
Use: For the isolation and presumptive identification of Yersinia
enterocolitica and Yersinia pseudotuberculosis from other fermenting
Gram-negative bacilli such as Enterocolitica agglomerans which is
often confused with Yersinia. Enterocolitica agglomerans does not
produce pectinase. Yersinia enterocolitica is strongly positive for pec-
tinase activity. Yersinia pseudotuberculosis is weakly positive for pec-
tinase activity. Yersinia pestis is negative for pectinase activity. Also
used for the differentiation of Klebsiella oxytoca, which is pectinase
positive.