Pea Agar Medium

[所属分类:培养基配方] [发布时间:2021-6-30] [发布人:网站管理员2] [阅读次数:] [返回]

Pea Agar Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 3.0L:

Dry peas....................................................................................360.0g
Agar ............................................................................................45.0g
Preparation of Medium: Add dry peas to 2.2L of distilled/deion-
ized water. Autoclave for 2 hr at 15 psi pressure–121°C. Filter pea mash
through cheesecloth. Reserve filtrate. Bring volume of filtrate to 3.0L
with distilled/deionized water. Mix thoroughly. Add agar. Gently heat
and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Allow tubes to cool in a slanted position.
Use: For the cultivation and maintenance of Ascochyta pinodes, Asco-
chyta pisi, and Phoma medicaginis.