PE2 Medium

[所属分类:培养基配方] [发布时间:2021-6-30] [发布人:网站管理员2] [阅读次数:] [返回]

PE2 Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Peptone .......................................................................................20.0g
Yeast extract..................................................................................3.0g
Alaska seed peas....................................................................416–520
Bromcresol Purple solution .......................................................2.0mL
Bromcresol Purple Solution:
Composition per 100.0mL:
Bromcresol Purple ........................................................................2.0g
Ethanol.....................................................................................10.0mL
Preparation of Bromcresol Purple Solution:  Add Bromcresol
Purple to 10.0mL of ethanol. Mix thoroughly. Bring volume to
100.0mL with distilled/deionized water. Filter sterilize.
Preparation of Medium: Add components, except Alaska seed
peas, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Gently heat until dissolved. Add 8–10 Alaska seed peas to each
of 18 × 150 mm screw-capped tubes. Distribute the broth into each tube
in 19.0mL volumes. Allow tubes to stand for 1 hr. Autoclave for 15 min
at 15 psi pressure–121°C.
Use: For the cultivation of Clostridium botulinum from foods.