PE-2 HiVeg Medium

[所属分类:培养基配方] [发布时间:2021-6-30] [发布人:网站管理员2] [阅读次数:] [返回]

PE-2 HiVeg Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Plant peptone ..............................................................................20.0g
Yeast extract..................................................................................3.0g
Bromcresol Purple......................................................................0.04g
Alaska seed peas..................................................................... variable
pH 6.8 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components, except alaska seed
peas, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Gently heat and bring to boiling. Distribute into screw-cap
tubes. Add 8–10 untreated Alaska seed peas per tube and let the tubes
stand for 1 hr to permit hydration. Autoclave for 15 min at 15 psi pres-
sure–121°C.
Use: For the cultivation of Clostridium botulinum from foods.