PCA+T+T

[所属分类:培养基配方] [发布时间:2021-6-30] [发布人:网站管理员2] [阅读次数:] [返回]

PCA+T+T

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Potatoes.....................................................................................300.0g
Carrots.........................................................................................25.0g
Agar ............................................................................................15.0g
β-sitosterol...............................................................................30.0mg
Tryptophan...............................................................................20.0mg
Preparation of Medium: Slice potatoes with skin. Peel and slice
carrots. Place 300.0g of potatoes and 25.0g of carrots in 1.0L of dis-
tilled/deionized water. Gently heat and bring to boiling. Allow to boil
for 20 min. Filter through Whatman filer paper. Add agar and other
components to filtrate. Bring volume to 1.0L with distilled/deionized
water. Mix thoroughly. Gently heat and bring to boiling. Distribute into
tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour
into sterile Petri dishes or leave in tubes.
Use:  For the cultivation of Phytophthora nicotianae, Phytophthora
citrophthora, and Phytophthora cactorum.