PD2 Medium

[所属分类:培养基配方] [发布时间:2021-6-30] [发布人:网站管理员2] [阅读次数:] [返回]

PD2 Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Pancreatic digest of casein............................................................4.0g
Papaic digest of soybean meal......................................................2.0g
K 2 HPO 4 .........................................................................................1.5g
Disodium succinate.......................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................1.0g
Trisodium citrate...........................................................................1.0g
Bovine serum albumin solution...............................................10.0mL
Hemin chloride solution...........................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Bovine Serum Albumin Solution:
Composition per 10.0mL:
Bovine serum albumin..................................................................2.0g
Preparation of Bovine Serum Albumin Solution: Add bovine
serum albumin to distilled/deionized water and bring volume to
10.0mL. Mix thoroughly. Filter sterilize.
Hemin Chloride Solution:
Composition per 100.0mL:
Hemin chloride .............................................................................0.1g
NaOH (0.05N solution)..........................................................100.0mL
Preparation of Hemin Chloride Solution: Add hemin chloride
to 100.0mL of NaOH solution. Mix thoroughly.
Preparation of Medium: Add components, except bovine serum
albumin solution, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Gently heat and bring to boiling. Adjust pH
to 7.0. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add sterile bovine serum albumin solution. Mix thor-
oughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of PD-ALS (Pierce’s disease-
almond leaf scorch) bacteria.