PCA

[所属分类:培养基配方] [发布时间:2021-6-30] [发布人:网站管理员2] [阅读次数:] [返回]

PCA

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Potatoes.....................................................................................300.0g
Carrots.........................................................................................25.0g
Agar............................................................................................15.0g
Preparation of Medium: Slice potatoes with skin. Peel and slice
carrots. Place 300.0g of potatoes and 25.0g of carrots in 1.0L of dis-
tilled/deionized water. Gently heat and bring to boiling. Allow to boil
for 20 min. Filter through Whatman filer paper. Add agar to filtrate.
Bring volume to 1.0 L with distilled/deionized water. Mix thoroughly.
Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes
or leave in tubes.
Use:  For the cultivation of Cochliobolus ravenelii, Humicola fuscoa-
tra, Humicola grisea, Pyrenophora tritici-repentis, and Verticillium
fungicola.