Park and Sanders Enrichment Broth

[所属分类:培养基配方] [发布时间:2021-6-30] [发布人:网站管理员2] [阅读次数:] [返回]

Park and Sanders Enrichment Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1010.0mL:

Basal medium ...............................................................................1.0L
Supplement A ............................................................................5.0mL
Supplement B.............................................................................5.0mL
pH 7.0 ± 0.2 at 25°C
Basal Medium:
Composition per liter:
Pancreatic digest of casein..........................................................10.0g
Peptic digest of animal tissue......................................................10.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................2.0g
Glucose .........................................................................................1.0g
Sodium pyruvate.........................................................................0.25g
NaHSO 3 ........................................................................................0.1g
Horse blood, lysed ...................................................................50.0mL
Preparation of Basal Medium: Add components, except horse
blood, to distilled/deionized water and bring volume to 950.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 25°C. Aseptically add sterile horse
blood. Mix thoroughly.
Supplement A:
Composition per 5.0mL:
Vancomycin ................................................................................0.01g
Trimethoprim lactate...................................................................0.01g
Preparation of Supplement A: Add components to distilled/de-
ionized water and bring volume to 5.0mL. Mix thoroughly. Filter ster-
ilize.
Supplement B:
Composition per 5.0mL:
Cefoperazone............................................................................0.032g
Cycloheximide..............................................................................0.1g
Preparation of Supplement B: Add components to distilled/de-
ionized water and bring volume to 5.0mL. Mix thoroughly. Filter ster-
ilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium:  To 1.0L of cooled, sterile basal medium,
aseptically add 5.0mL of sterile supplement A. Mix thoroughly. Asep-
tically distribute into flasks in 100.0mL volumes. Inoculate medium
with food samples. Incubate at 31°–32°C for 4 hr to recover and resus-
citate injured cells. Aseptically add 0.5mL of supplement B to each
100.0mL of medium. Incubate cultures at 37°C for 2 hr.
Use: For the cultivation and enrichment of Campylobacter species
from foods.