Paramecium Medium

[所属分类:培养基配方] [发布时间:2021-6-30] [发布人:网站管理员2] [阅读次数:] [返回]

Paramecium Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Solution C..............................................................................500.0mL
Solution A................................................................................10.0mL
Solution B..................................................................................1.0mL
Solution A:
Composition per liter:
Thiamine·HCl ...............................................................................1.5g
Calcium pantothenate ...................................................................1.0g
Nicotinamide.................................................................................0.5g
Pyridoxal·HCl...............................................................................0.5g
Riboflavin .....................................................................................0.5g
Folic acid.......................................................................................0.5g
α-Lipoic acid.................................................................................0.1g
Biotin .........................................................................................0.1mg
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute while stir-
ring into screw-capped tubes in 10.0mL volumes. Store at −20°C.
Thaw as needed.
Solution B:
Composition per 100.0mL:
TEM-4T (Hachmeister, Pittsburgh)............................................10.0g
Stigmasterol ..................................................................................0.5g
Ethanol, absolute....................................................................100.0mL
Preparation of Solution B: Add TEM-4T and stigmasterol to
100.0mL of hot ethanol. Mix thoroughly. Store at 4°C.
Solution C:
Composition per 500.0mL:
Proteose peptone.........................................................................10.0g
Pancreatic digest of casein............................................................5.0g
Ribonucleic acid ...........................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.5g
Preparation of Solution C: Add components to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly.
Preparation of Medium: Combine 500.0mL of solution C, 10.0mL
of solution A, and 1.0mL of solution B. Mix thoroughly. Bring volume
to 1.0L with distilled/deionized water. Adjust pH to 7.0–7.2 with 0.1N
NaOH. Autoclave for 20 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Paramecium species to be used as host cells
by bacterial symbionts.