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Park and Sanders Enrichment HiVeg Broth Base with Horse Blood and Selective Antibiotics
[所属分类:培养基配方] [发布时间:2021-6-30] [发布人:网站管理员2] [阅读次数:] [返回]
Park and Sanders Enrichment HiVeg Broth Base
with Horse Blood and Selective Antibiotics
Plant peptone ..............................................................................10.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................2.0g
Glucose.........................................................................................1.0g
Sodium pyruvate.........................................................................0.25g
Sodium biselenite..........................................................................0.1g
Horse blood, lysed...................................................................50.0mL
Supplement A............................................................................5.0mL
Supplement B.............................................................................5.0mL
pH 7.0 ± 0.2 at 25°C
Source: This medium, without horse blood and selective antibiotic
supplements, is available as a premixed powder from HiMedia.
Supplement A:
Composition per 5.0mL:
Vancomycin................................................................................0.01g
Trimethoprim lactate...................................................................0.01g
Preparation of Supplement A: Add components to distilled/de-
ionized water and bring volume to 5.0mL. Mix thoroughly. Filter ster-
ilize.
Supplement B:
Composition per 5.0mL:
Cefoperazone............................................................................0.032g
Cycloheximide..............................................................................0.1g
Preparation of Supplement B: Add components to distilled/de-
ionized water and bring volume to 5.0mL. Mix thoroughly. Filter ster-
ilize.
Caution: Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components, except horse blood,
supplement A and supplement B, to distilled/deionized water and bring
volume to 940.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Asepti-
cally add 50.0mL sterile horse blood. Mix thoroughly. Aseptically add
5.0mL of sterile supplement A. Mix thoroughly. Aseptically distribute
into flasks in 100.0mL volumes. Inoculate medium with food samples.
Incubate at 31°–32°C for 4 hr to recover and resuscitate injured cells.
Aseptically add 0.5mL of supplement B to each 100.0mL of medium.
Incubate cultures at 37°C for 2 hr.
Use: For the cultivation and enrichment of Campylobacter species
from foods.
with Horse Blood and Selective Antibiotics
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Plant hydrolysate ........................................................................10.0gPlant peptone ..............................................................................10.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................2.0g
Glucose.........................................................................................1.0g
Sodium pyruvate.........................................................................0.25g
Sodium biselenite..........................................................................0.1g
Horse blood, lysed...................................................................50.0mL
Supplement A............................................................................5.0mL
Supplement B.............................................................................5.0mL
pH 7.0 ± 0.2 at 25°C
Source: This medium, without horse blood and selective antibiotic
supplements, is available as a premixed powder from HiMedia.
Supplement A:
Composition per 5.0mL:
Vancomycin................................................................................0.01g
Trimethoprim lactate...................................................................0.01g
Preparation of Supplement A: Add components to distilled/de-
ionized water and bring volume to 5.0mL. Mix thoroughly. Filter ster-
ilize.
Supplement B:
Composition per 5.0mL:
Cefoperazone............................................................................0.032g
Cycloheximide..............................................................................0.1g
Preparation of Supplement B: Add components to distilled/de-
ionized water and bring volume to 5.0mL. Mix thoroughly. Filter ster-
ilize.
Caution: Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components, except horse blood,
supplement A and supplement B, to distilled/deionized water and bring
volume to 940.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Asepti-
cally add 50.0mL sterile horse blood. Mix thoroughly. Aseptically add
5.0mL of sterile supplement A. Mix thoroughly. Aseptically distribute
into flasks in 100.0mL volumes. Inoculate medium with food samples.
Incubate at 31°–32°C for 4 hr to recover and resuscitate injured cells.
Aseptically add 0.5mL of supplement B to each 100.0mL of medium.
Incubate cultures at 37°C for 2 hr.
Use: For the cultivation and enrichment of Campylobacter species
from foods.
