Paraffin Medium with McClung Carbon-Free Broth

[所属分类:培养基配方] [发布时间:2021-6-30] [发布人:网站管理员2] [阅读次数:] [返回]

Paraffin Medium with McClung Carbon-Free Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Paraffin pellets................................................................................1lb
McClung carbon-free broth ..........................................................1.0L
pH 7.2 ± 0.2 at 25°C
McClung Carbon-Free Broth:
Composition per liter:
NaNO 3 ..........................................................................................2.0g
K 2 HPO 4 .........................................................................................0.8g
MgSO 4 ·7H 2 O................................................................................0.5g
FeCl 3 ...........................................................................................0.01g
MnCl 2 ·4H 2 O .............................................................................0.008g
ZnSO 4 .......................................................................................0.002g
Preparation of McClung Carbon-Free Broth : Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat (low heat for 15–30 min) until salts dissolve. Cool to 25°C.
Adjust pH to 7.2, if necessary. Filter sterilize.
Preparation of Medium: Fill glass screw-capped tubes 60% full
with paraffin pellets. Place on slanted rack and autoclave for 15 min at
121°C. Let tubes cool in a slanted position. Add 2.5mL of sterile Mc-
Clung carbon-free broth to each paraffin slant. Tighten screw caps. Ste-
rility is tested by the addition of 2.5mL of sterile Trypticase™ soy
broth and sample to each slant.
Use: For use in the sterility testing of various specimens.