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培养基配方
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Paraffin Agar
[所属分类:培养基配方] [发布时间:2021-6-30] [发布人:网站管理员2] [阅读次数:] [返回]
Paraffin Agar
K 2 HPO 4 .........................................................................................6.0g
NH 4 NO 3 ........................................................................................4.0g
KH 2 PO 4 .........................................................................................2.0g
Paraffin, liquid..............................................................................1.0g
ZnSO 4 ·7H 2 O.............................................................................0.049g
MnCl 2 ·4H 2 O .............................................................................0.046g
FeSO 4 ·7H 2 O...............................................................................5.4mg
CuSO 4 ·5H 2 O..............................................................................2.5mg
Na 2 B 4 O 7 ·10H 2 O.......................................................................0.94mg
(NH 4 ) 6 Mo 7 O 24 ·4H 2 O.................................................................0.2mg
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the selective isolation and cultivation of streptomycetes.
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Agar............................................................................................15.0gK 2 HPO 4 .........................................................................................6.0g
NH 4 NO 3 ........................................................................................4.0g
KH 2 PO 4 .........................................................................................2.0g
Paraffin, liquid..............................................................................1.0g
ZnSO 4 ·7H 2 O.............................................................................0.049g
MnCl 2 ·4H 2 O .............................................................................0.046g
FeSO 4 ·7H 2 O...............................................................................5.4mg
CuSO 4 ·5H 2 O..............................................................................2.5mg
Na 2 B 4 O 7 ·10H 2 O.......................................................................0.94mg
(NH 4 ) 6 Mo 7 O 24 ·4H 2 O.................................................................0.2mg
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the selective isolation and cultivation of streptomycetes.