Paracoccus kocurii Medium (DSMZ Medium 773)

[所属分类:培养基配方] [发布时间:2021-6-30] [发布人:网站管理员2] [阅读次数:] [返回]

Paracoccus kocurii Medium
(DSMZ Medium 773)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaH 2 PO 4 ·2H 2 O...........................................................................1.71g
K 2 HPO 4 .......................................................................................1.41g
(NH 4 ) 2 SO 4 .....................................................................................1.0g
Hutner's salt solution................................................................20.0mL
Tetramethyl ammonium chloride solution...............................10.0mL
Thiamine hydrochloride solution.............................................10.0mL
pH 6.9 ± 0.2 at 25°C
Tetramethyl Ammonium Chloride Solution:
Composition per 10.0mL:
Tetramethyl ammonium chloride..................................................1.0g
Preparation of Tetramethyl Ammonium Chloride Solution:
Add tetramethyl ammonium chloride to distilled/deionized water and
bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Thiamine Hydrochloride Solution:
Composition per 10.0mL:
Thiamine-HCl·2H 2 O..................................................................0.5mg
Preparation of Thiamine Hydrochloride Solution:  Add thia-
mine-HCl·2H 2 O to distilled/deionized water and bring volume to
10.0mL. Mix thoroughly. Filter sterilize.
Hutner’s Salt Solution:
Composition per liter:
MgSO 4 ·7H 2 O..............................................................................29.7g
Nitrilotriacetic acid.....................................................................10.0g
CaCl 2 ·2H 2 O...............................................................................3.335g
FeSO 4 ·7H 2 O.............................................................................99.0mg
(NH 4 ) 6 MoO 7 O 24 ·4H 2 O ............................................................9.25mg
"Metals 44" ..............................................................................50.0mL
"Metals 44":
Composition per 100.0mL:
ZnSO 4 ·7H 2 O.............................................................................1.095g
FeSO 4 ·7H 2 O..................................................................................0.5g
Sodium EDTA.............................................................................0.25g
MnSO 4 ·H2O..............................................................................0.154g
CuSO 4 ·5H 2 O............................................................................39.2mg
Co(NO 3 ) 2 ·6H 2 O .......................................................................24.8mg
Na 2 B 4 O 7 ·10H 2 O.......................................................................17.7mg
Preparation of “Metals 44”: Add sodium EDTA to distilled/deion-
ized water and bring volume to 90.0mL. Mix thoroughly. Add a few drops
of concentrated H 2 SO 4 to retard precipitation of heavy metal ions. Add re-
maining components. Mix thoroughly. Bring volume to 100.0mL with dis-
tilled/deionized water.
Preparation of Hutner’s Salt Solution:  Add nitrilotriacetic acid
to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with KOH.
Add remaining components. Add distilled/deionized water to 1.0L.
Adjust pH to 6.8.
Preparation of Medium: Add components, except tetramethyl am-
monium chloride solution and thiamine hydrochloride solution, to dis-
tilled/deionized water and bring volume to 980.0L. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to room tempera-
ture. Aseptically add 10.0mL sterile tetramethyl ammonium chloride
solution and 10.0mL sterile thiamine hydrochloride solution. Mix thor-
oughly. Adjust pH to 6.9.
Use:  For the cultivation of Paracoccus kocurii.