Pantothenate Medium, AOAC USP

[所属分类:培养基配方] [发布时间:2021-6-30] [发布人:网站管理员2] [阅读次数:] [返回]

Pantothenate Medium, AOAC USP

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose.......................................................................................40.0g
Sodium acetate............................................................................20.0g
Vitamin assay casamino acids ....................................................10.0g
K 2 HPO 4 .........................................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
L- Cystine.......................................................................................0.4g
MgSO 4 ·7H 2 O................................................................................0.4g
L- Tryptophan.................................................................................0.1g
Sorbitan monooleate complex ......................................................0.1g
Adenine sulfate...........................................................................0.02g
FeSO 4 ·7H 2 O................................................................................0.02g
Guanine·HCl...............................................................................0.02g
MnSO 4 ·H 2 O................................................................................0.02g
NaCl............................................................................................0.02g
Uracil..........................................................................................0.02g
Nicotinic acid.............................................................................1.0mg
Pyridoxine·HCl..........................................................................0.8mg
Riboflavin..................................................................................0.4mg
p-Aminobenzoic acid.................................................................0.2mg
Thiamine·HCl............................................................................0.2mg
Biotin..........................................................................................0.8μg
pH 6.7 ± 0.1 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Gently heat and
bring to boiling. Continue boiling for 2–3 min. Distribute into tubes in
5.0mL volumes. Add standard solution or test solution to each tube.
Adjust the volume of each tube to 10.0mL with distilled/deionized wa-
ter. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the determination of pantothenic acid and its salts using Lac-
tobacillus plantarum as the test organism.