Panthenol Assay Medium

[所属分类:培养基配方] [发布时间:2021-6-30] [发布人:网站管理员2] [阅读次数:] [返回]

Panthenol Assay Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 950.0mL:

Panthenol supplement............................................................475.0mL
Base medium..........................................................................225.0mL
pH 6.0 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Base Medium:
Composition per 900.0mL:
Glucose .......................................................................................15.0g
Pancreatic digest of casein, charcoal treated...............................10.0g
Sodium citrate...............................................................................2.0g
Vitamin assay casamino acids.......................................................2.0g
K 2 HPO 4 .........................................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.8g
L- Tryptophan.................................................................................0.2g
MnSO 4 ·H 2 O................................................................................0.16g
L- Cystine .....................................................................................0.15g
FeSO 4 ·7H 2 O................................................................................0.04g
Liver concentrate ........................................................................0.04g
NaCl............................................................................................0.04g
Adenine sulfate...........................................................................0.01g
Guanine·HCl...............................................................................0.01g
Uracil ..........................................................................................0.01g
p-Aminobenzoic acid.................................................................2.0mg
β-Alanine...................................................................................2.0mg
Nicotinic acid.............................................................................2.0mg
Pyridoxine·HCl..........................................................................2.0mg
Riboflavin ..................................................................................2.0mg
Thiamine·HCl ............................................................................2.0mg
Folic acid..................................................................................0.02mg
Biotin .....................................................................................0.016mg
Preparation of Base Medium:  Add components to distilled/de-
ionized water and bring volume to 900.0mL. Mix thoroughly. Gently
heat and bring to boiling. Mix thoroughly.
Panthenol Supplement:
Composition per 100.0mL:
Glycerol ......................................................................................33.0g
Sorbitan monooleate complex ......................................................2.0g
Lactic acid...................................................................................0.68g
Preparation of Panthenol Supplement: Add components to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Distribute base medium into 50.0mL
flasks in 4.5mL volumes. Add standard solution or test solution to each
flask. Adjust the volume in each flask to 9.5mL with distilled/deion-
ized water. Autoclave for 10 min at 15 psi pressure–121°C. Cool to
25°C. Aseptically add 0.5mL of panthenol supplement to each flask.
Use: For the microbiological assaying of panthenol using Glu-
conobacter oxydans subspecies suboxydans as the test organism.