Pantothenate Inoculum HiVeg Broth

[所属分类:培养基配方] [发布时间:2021-6-30] [发布人:网站管理员2] [阅读次数:] [返回]

Pantothenate Inoculum HiVeg Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Plant hydrolysate No. 4...............................................................15.0g
Glucose.......................................................................................10.0g
Tomato juice (100 ml) ..................................................................5.0g
Yeast extract..................................................................................5.0g
KH 2 PO 4 .........................................................................................2.0g
Polysorbate 80 ..............................................................................1.0g
pH 6.8 ± 0.1 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Gently heat and
bring to boiling. Continue boiling for 2–3 min. Distribute into tubes in
5.0mL volumes. Add standard solution or test solution to each tube.
Adjust the volume of each tube to 10.0mL with distilled/deionized wa-
ter. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the microbiological assaying of pantothenic acid and its salts
using Lactobacillus plantarum as the test organism.