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PALCAM Agar (Polymyxin Acriflavine Lithium Chloride Ceftazidime Esculin Mannitol Agar)

[所属分类:培养基配方] [发布时间:2021-6-29] [发布人:网站管理员2] [阅读次数:] [返回]

PALCAM Agar
(Polymyxin Acriflavine Lithium Chloride
Ceftazidime Esculin Mannitol Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter :

Peptone .......................................................................................23.0g
LiCl 2 ...........................................................................................15.0g
Agar............................................................................................10.0g
Mannitol......................................................................................10.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................3.0g
Starch............................................................................................1.0g
Esculin..........................................................................................0.8g
Ferric ammonium citrate...............................................................0.5g
Glucose.........................................................................................0.5g
Phenol Red..................................................................................0.08g
PALCAM selective supplement...............................................10.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Oxoid
Unipath.
PALCAM Selective Supplement:
Composition per 10.0mL:
Ceftazidime..............................................................................20.0mg
Polymyxin B............................................................................10.0mg
Acriflavine·HCl .........................................................................5.0mg
Preparation of PALCAM Selective Supplement: Add compo-
nents to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, except PALCAM se-
lective supplement, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add sterile PALCAM selective supplement. Mix thoroughly.
Use: For the selective isolation, cultivation, and differentiation of List-
eria monocytogenes and other Listeria species from foods.

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PALCAM Agar (Polymyxin Acriflavine Lithium Chloride Ceftazidime Esculin Mannitol Agar)