Pai Medium

[所属分类:培养基配方] [发布时间:2021-6-29] [发布人:网站管理员2] [阅读次数:] [返回]

Pai Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1120.0mL:

Glucose.........................................................................................5.0g
Homogenized whole egg ..............................................................1.0L
Glycerol .................................................................................120.0mL
pH 6.75 ± 0.2 at 25°C
Homogenized Whole Egg:
Composition per liter:
Whole eggs................................................................................18–24
Preparation of Homogenized Whole Egg:  Use fresh eggs, less
than 1 week old. Scrub the shells with soap. Let stand in a soap solution
for 30 min. Rinse in running water. Soak eggs in 70% ethanol for 15
min. Break the eggs into a sterile container. Homogenize by shaking.
Filter through four layers of sterile cheesecloth into a sterile graduated
cylinder. Measure out 1.0L.
Preparation of Medium: Combine components. Mix thoroughly.
Aseptically distribute into sterile tubes. Inspissate tubes in a slanted po-
sition at 80°–90°C (moist heat) for 30 min.
Use: For the isolation and cultivation of Corynebacterium spp.