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PALCAM Agar with Egg Yolk Emulsion (Polymyxin Acriflavine Lithium Chloride Ceftazidime Esculin Mannitol Agar with Egg Yolk Emulsion)
[所属分类:培养基配方] [发布时间:2021-6-29] [发布人:网站管理员2] [阅读次数:] [返回]
PALCAM Agar with Egg Yolk Emulsion
(Polymyxin Acriflavine Lithium Chloride Ceftazidime
Esculin Mannitol Agar with Egg Yolk Emulsion)
LiCl 2 ............................................................................................15.0g
Agar ............................................................................................10.0g
Mannitol......................................................................................10.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................3.0g
Starch ............................................................................................1.0g
Esculin ..........................................................................................0.8g
Ferric ammonium citrate...............................................................0.5g
Glucose .........................................................................................0.5g
Phenol Red..................................................................................0.08g
Egg yolk emulsion...................................................................25.0mL
PALCAM selective supplement...............................................10.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Oxoid
Unipath.
PALCAM Selective Supplement:
Composition per 10.0mL:
Ceftazidime..............................................................................20.0mg
Polymyxin B............................................................................10.0mg
Acriflavine·HCl..........................................................................5.0mg
Preparation of PALCAM Selective Supplement: Add compo-
nents to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Egg Yolk Emulsion:
Composition :
Chicken egg yolks............................................................................11
Whole chicken egg.............................................................................1
Preparation of Egg Yolk Emulsion: Soak eggs with 1:100 dilu-
tion of saturated mercuric chloride solution for 1 min. Crack eggs and
separate yolks from whites. Mix egg yolks with 1 chicken egg.
Preparation of Medium: Add components, except PALCAM se-
lective supplement and egg yolk emulsion, to distilled/deionized water
and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. Aseptically add sterile PALCAM selective supplement and
egg yolk emulsion. Mix thoroughly. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use: For the selective isolation, cultivation, and differentiation of List-
eria monocytogenes and other Listeria species from foods The addition
of egg yolk emulsion aids in the recovery of damaged Listeria.
(Polymyxin Acriflavine Lithium Chloride Ceftazidime
Esculin Mannitol Agar with Egg Yolk Emulsion)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter :
Peptone........................................................................................23.0gLiCl 2 ............................................................................................15.0g
Agar ............................................................................................10.0g
Mannitol......................................................................................10.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................3.0g
Starch ............................................................................................1.0g
Esculin ..........................................................................................0.8g
Ferric ammonium citrate...............................................................0.5g
Glucose .........................................................................................0.5g
Phenol Red..................................................................................0.08g
Egg yolk emulsion...................................................................25.0mL
PALCAM selective supplement...............................................10.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Oxoid
Unipath.
PALCAM Selective Supplement:
Composition per 10.0mL:
Ceftazidime..............................................................................20.0mg
Polymyxin B............................................................................10.0mg
Acriflavine·HCl..........................................................................5.0mg
Preparation of PALCAM Selective Supplement: Add compo-
nents to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Egg Yolk Emulsion:
Composition :
Chicken egg yolks............................................................................11
Whole chicken egg.............................................................................1
Preparation of Egg Yolk Emulsion: Soak eggs with 1:100 dilu-
tion of saturated mercuric chloride solution for 1 min. Crack eggs and
separate yolks from whites. Mix egg yolks with 1 chicken egg.
Preparation of Medium: Add components, except PALCAM se-
lective supplement and egg yolk emulsion, to distilled/deionized water
and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. Aseptically add sterile PALCAM selective supplement and
egg yolk emulsion. Mix thoroughly. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use: For the selective isolation, cultivation, and differentiation of List-
eria monocytogenes and other Listeria species from foods The addition
of egg yolk emulsion aids in the recovery of damaged Listeria.
