Oxidation-Fermentation Medium (OF Medium)

[所属分类:培养基配方] [发布时间:2021-6-29] [发布人:网站管理员2] [阅读次数:] [返回]

Oxidation-Fermentation Medium
(OF Medium)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..............................................................................................5.0g
Agar ..............................................................................................2.5g
Pancreatic digest of casein............................................................2.0g
K 2 HPO 4 .........................................................................................0.3g
Bromthymol Blue .......................................................................0.03g
Carbohydrate solution............................................................100.0mL
pH 6.8 ± 0.1 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Carbohydrate Solution:
Composition per 100.0mL:
Carbohydrate...............................................................................10.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except carbohydrate
solution, to distilled/deionized water and bring volume to 900.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 100.0mL
of sterile carbohydrate solution. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For differentiating Gram-negative bacteria based upon determin-
ing the oxidative and fermentative metabolism of carbohydrates.