Oxford Medium (BAM M118)

[所属分类:培养基配方] [发布时间:2021-6-29] [发布人:网站管理员2] [阅读次数:] [返回]

Oxford Medium
(BAM M118)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Special peptone...........................................................................23.0g
LiCl.............................................................................................15.0g
Agar ............................................................................................10.0g
NaCl..............................................................................................5.0g
Cornstarch.....................................................................................1.0g
Esculin ..........................................................................................1.0g
Ferric ammonium citrate...............................................................0.5g
Antibiotic inhibitor ..................................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Caution: Lithium chloride is harmful. Avoid bodily contact and inha-
lation of vapors. On contact with skin, wash with plenty of water im-
mediately.
Supplement Mix:
Composition per 10.0mL:
Cycloheximide..............................................................................0.4g
Colistin sulfate............................................................................0.02g
Fosfomycin .................................................................................0.01g
Acriflavine.................................................................................5.0mg
Cefotetan....................................................................................2.0mg
Ethanol (50% solution)............................................................10.0mL
Preparation of Supplement Mix: Add components to 10.0mL of
ethanol. Mix thoroughly. Filter sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components, except supplement
mix, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 50°C. Aseptically add 10.0mL of ster-
ile supplement mix. Mix thoroughly. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use : For the isolation and cultivation of Listeria monocytogenes from
specimens containing a mixed bacterial flora.