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Oxidation-Fermentation Medium, Hugh-Leifson’s (Hugh-Leifson’s Oxidation Fermentation Medium)
[所属分类:培养基配方] [发布时间:2021-6-29] [发布人:网站管理员2] [阅读次数:] [返回]
Oxidation-Fermentation Medium, Hugh-Leifson’s
(Hugh-Leifson’s Oxidation Fermentation Medium)
Agar..............................................................................................3.0g
Peptone .........................................................................................2.0g
K 2 HPO 4 .........................................................................................0.3g
Carbohydrate solution............................................................100.0mL
Bromthymol Blue solution (0.2% ) .........................................15.0mL
pH 7.1 ± 0.2 at 25°C
Carbohydrate Solution:
Composition per 100.0mL:
Carbohydrate...............................................................................10.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except carbohydrate
solution, to distilled/deionized water and bring volume to 900.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 100.0mL
of sterile carbohydrate solution. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For differentiating Gram-negative bacteria, such as Vibrio species,
based upon determining the oxidative and fermentative metabolism of car-
bohydrates. Bacteria that ferment the carbohydrate turn the medium yel-
low.
(Hugh-Leifson’s Oxidation Fermentation Medium)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
NaCl..............................................................................................5.0gAgar..............................................................................................3.0g
Peptone .........................................................................................2.0g
K 2 HPO 4 .........................................................................................0.3g
Carbohydrate solution............................................................100.0mL
Bromthymol Blue solution (0.2% ) .........................................15.0mL
pH 7.1 ± 0.2 at 25°C
Carbohydrate Solution:
Composition per 100.0mL:
Carbohydrate...............................................................................10.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except carbohydrate
solution, to distilled/deionized water and bring volume to 900.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 100.0mL
of sterile carbohydrate solution. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For differentiating Gram-negative bacteria, such as Vibrio species,
based upon determining the oxidative and fermentative metabolism of car-
bohydrates. Bacteria that ferment the carbohydrate turn the medium yel-
low.
