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培养基配方
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Oxford Agar, Modified (Listeria Selective Agar, Modified Oxford) (MOX Agar)
[所属分类:培养基配方] [发布时间:2021-6-29] [发布人:网站管理员2] [阅读次数:] [返回]
Oxford Agar, Modified
(Listeria Selective Agar, Modified Oxford)
(MOX Agar)
LiCl.............................................................................................15.0g
Agar............................................................................................12.0g
NaCl..............................................................................................5.0g
Cornstarch.....................................................................................1.0g
Esculin ..........................................................................................1.0g
Ferric ammonium citrate...............................................................0.5g
Antibiotic inhibitor..................................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Caution: Lithium chloride is harmful. Avoid bodily contact and inha-
lation of vapors. On contact with skin, wash with plenty of water im-
mediately.
Antibiotic Inhibitor:
Composition per 10.0mL:
Moxalactam ..............................................................................0.015g
Colistin sulfate............................................................................0.01g
Preparation of Antibiotic Inhibitor: Add components to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except antibiotic inhib-
itor, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 10 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of
sterile antibiotic inhibitor. Mix thoroughly. Pour into sterile Petri dish-
es or distribute into sterile tubes.
Use: For the isolation and cultivation of Listeria monocytogenes from
specimens containing a mixed bacterial flora.
(Listeria Selective Agar, Modified Oxford)
(MOX Agar)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Special peptone...........................................................................23.0gLiCl.............................................................................................15.0g
Agar............................................................................................12.0g
NaCl..............................................................................................5.0g
Cornstarch.....................................................................................1.0g
Esculin ..........................................................................................1.0g
Ferric ammonium citrate...............................................................0.5g
Antibiotic inhibitor..................................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Caution: Lithium chloride is harmful. Avoid bodily contact and inha-
lation of vapors. On contact with skin, wash with plenty of water im-
mediately.
Antibiotic Inhibitor:
Composition per 10.0mL:
Moxalactam ..............................................................................0.015g
Colistin sulfate............................................................................0.01g
Preparation of Antibiotic Inhibitor: Add components to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except antibiotic inhib-
itor, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 10 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of
sterile antibiotic inhibitor. Mix thoroughly. Pour into sterile Petri dish-
es or distribute into sterile tubes.
Use: For the isolation and cultivation of Listeria monocytogenes from
specimens containing a mixed bacterial flora.
