ESA Medium (Epoxysuccinic Acid Medium)

[所属分类:培养基配方] [发布时间:2020-7-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

ESA Medium
(Epoxysuccinic Acid Medium)

培养基配方
Composition per liter:
Agar .......................................................................20.0g
(NH4)2SO4 ...................................................................3.0g
KH2PO4 ......................................................................1.5g
Na2HPO4 .....................................................................1.5g
MgSO4 ·7H2O.................................................................0.5g
Yeast extract................................................................0.5g
CaCl2 ·2H2O................................................................0.01g
FeSO4 ·7H2O................................................................0.01g
MnSO4 ·4H2O...............................................................0.001g
Epoxysuccinate solution...................................................100.0mL
pH 7.0 ± 0.2 at 25°C
Epoxysuccinate Solution:
Composition per 100.0mL:
Sodium cis-epoxysuccinate...................................................10.0g
Preparation of Epoxysuccinate Solution: Add sodium cis-epox-
ysuccinate to distilled/deionized water and bring volume to 100.0mL.
Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except epoxysuccinate
solution, to distilled/deionized water and bring volume to 900.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 50°–60°C. Aseptically add sterile
epoxysuccinate solution. Mix thoroughly. Pour into sterile Petri dishes
or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Nocardia tartaricans.