Escherichia Medium (ATCC Medium 53)

[所属分类:培养基配方] [发布时间:2020-7-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Escherichia Medium
(ATCC Medium 53)

培养基配方
Composition per liter:
Agar..........................................................................15.0g
Casein hydrolysate.............................................................6.0g
K2HPO4 ........................................................................0.2g
MgSO4 ·7H2O...................................................................0.2g
Asparagine....................................................................0.15g
FeSO4 ·7H2O.................................................................1.0μg
Vitamin B 12 solution........................................................10.0mL
Glycerol .....................................................................2.0mL
pH 7.0 ± 0.2 at 25°C
Vitamin B 12 Solution:
Composition per 10.0mL:
Vitamin B 12 .................................................................0.04g
Preparation of Vitamin B 12 Solution: Add vitamin B 12 to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Preparation of Medium: Add components—except glycerol, agar,
and vitamin B 12 solution—to distilled/deionized water and bring vol-
ume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling.
Adjust pH to 7.0. Filter through Whatman #1 filter paper. Add glycerol
and agar. Mix thoroughly. Gently heat and bring to boiling. Add vita-
min B 12 solution. Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in
tubes.
Use:  For the cultivation and maintenance of Escherichia coli.