Erythromycin LB Medium

[所属分类:培养基配方] [发布时间:2020-7-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Erythromycin LB Medium

培养基配方
Composition per liter:
Pancreatic digest of casein.................................................10.0g
NaCl........................................................................10.0g
Yeast extract................................................................5.0g
Erythromycin solution .....................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Erythromycin Solution:
Composition per 10.0mL:
Erythromycin...............................................................50.0mg
Preparation of Erythromycin Solution: Add erythromycin to
distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except erythromycin
solution, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Bring pH to 7.0. Autoclave for 15 min at 15 psi pres-
sure–121°C. Aseptically add 10.0mL of sterile erythromycin solution.
Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Escherichia coli.