Erwinia Medium D3

[所属分类:培养基配方] [发布时间:2020-7-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Erwinia Medium D3

培养基配方
Composition per liter:
Agar ........................................................................15.0g
Arabinose....................................................................10.0g
Sucrose......................................................................10.0g
LiCl..........................................................................7.0g
Casein hydrolysate............................................................5.0g
NaCl..........................................................................5.0g
MgSO4 ·7H2O..................................................................0.3g
Acid Fuchsin..................................................................0.1g
Bromthymol Blue..............................................................0.06g
Sodium dodecyl sulfate.......................................................0.05g
pH 7.0 ± 0.2 at 25°C
Caution: Acid Fuchsin is a potential carcinogen and care must be tak-
en to avoid inhalation of the powdered dye and contact with the skin.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Adjust pH to 8.2. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C. The pH after autoclaving should be 7.0.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of Erwinia species.