Erwinia Selective Medium

[所属分类:培养基配方] [发布时间:2020-7-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Erwinia Selective Medium

培养基配方
Composition per liter:
Agar.........................................................................15.0g
(NH4)2SO4 ....................................................................5.0g
K2HPO4 .......................................................................2.0g
Eosin Y.......................................................................0.4g
Methylene Blue..............................................................0.065g
Glycerol ...................................................................10.0mL
Antibiotic solution.........................................................10.0mL
Antibiotic Solution:
Composition per 10.0mL:
Cycloheximide................................................................0.25g
Novobiocin ..................................................................0.04g
Neomycin sulfate.............................................................0.04g
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components, except antibiotic solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15
psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile antibiotic
solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the selective isolation and cultivation of Erwinia species.