Enterococcus Confirmatory HiVeg Agar with Penicillin

[所属分类:培养基配方] [发布时间:2020-7-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Enterococcus Confirmatory HiVeg Agar
with Penicillin

培养基配方
Composition per liter:
Agar..........................................................................15.0g
Glucose........................................................................5.0g
Plant hydrolysate .............................................................5.0g
Yeast extract..................................................................5.0g
NaN3 ..........................................................................0.4g
Methylene Blue................................................................0.01g
Penicillin solution..........................................................10.0mL
pH 8.0± 0.2 at 25°C
Source: This medium, without penicillin, is available as a premixed
powder from HiMedia.
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Penicillin Solution:
Composition per 10.0mL:
Penicillin....................................................................650U
Preparation of Penicillin Solution: Add penicillin to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except penicillin solu-
tion, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL sterile pen-
icillin solution. Mix thoroughly. Pour into sterile Petri dishes or dispense
into sterile tubes.
Use: For the isolation, cultivation, and enumeration of entercocci in
water, sewage, and feces by the membrane filter method. For the direct
plating of specimens for the detection and enumeration of fecal strep-
tococci.